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UNIMEDITREKHistopathology Knowledge Base
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Decalcification Quick Guide

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  1. Fix fully before decalcifying
  2. EDTA — gentle, antigen-friendly, slow
  3. Acid decalcifiers — faster, can harm antigens/DNA
  4. Check end-point (do not over-decalcify)
  5. Rinse well before processing
  6. Prefer EDTA when IHC / molecular is planned

Over-decalcification degrades staining and morphology.

Unimeditrek Pvt. Ltd. · www.unilabequipments.com · Educational template — adapt to your laboratory.

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