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Knowledge BaseTroubleshooting Atlas › Digital pathology

Tissue-detection misses parts of the section

What it looks like

Tissue sections appear incomplete or fragmented in the digital image.

Where it usually appears: Typically observed during digital scanning of histopathology slides.

Likely causes
  • Inadequate focus during scanning
  • Improper slide preparation
  • Inconsistent tissue thickness
  • Incorrect scanning parameters
Immediate checks
  • Verify focus settings on the scanner
  • Inspect the slide for physical damage
  • Check for air bubbles under the cover slip
Corrective action
  • Re-scan the slide with adjusted focus
  • Re-prepare the slide if necessary
  • Calibrate scanning equipment
Preventive action
  • Ensure consistent slide preparation techniques
  • Regularly maintain and calibrate scanning equipment
  • Train staff on proper scanning protocols
Equipment: Digital slide scannerRelated SOP: Digital Pathology Scanning SOP

This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.

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