Knowledge Base › Troubleshooting Atlas › Tissue processing
Small biopsies lost or damaged in processing
Also known as: lost biopsies, damaged samples
What it looks like
Small tissue fragments may appear missing or fragmented on the slide. Blocks may show uneven or incomplete sections.
Where it usually appears: Typically seen during routine processing of small biopsies, especially in automated processors.
Likely causes
- Inadequate fixation time
- Overheating during processing
- Improper embedding technique
- Inadequate sectioning
- Use of dull blades
Immediate checks
- Inspect the processing machine for jams
- Check fixation times for compliance
- Examine embedding molds for proper use
Corrective action
- Reprocess the samples if possible
- Use fresh embedding media
- Sharpen or replace microtome blades
Preventive action
- Ensure proper fixation protocols are followed
- Regular maintenance of processing equipment
- Train staff on embedding techniques
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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