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Knowledge BaseTroubleshooting Atlas › Floatation & mounting

Section placed on the wrong (back) side of the slide

What it looks like

The section appears inverted or not adhering properly to the slide, with potential loss of staining.

Where it usually appears: Typically appears during slide preparation or mounting processes.

Likely causes
  • Slide was flipped or rotated incorrectly during placement
  • Section adhered to the wrong side of the slide due to handling errors
Immediate checks
  • Verify the orientation of the section on the slide
  • Check for proper adherence of the section
Corrective action
  • Remove the section and re-mount it on the correct side of the slide
  • Ensure the section is properly oriented before mounting
Preventive action
  • Implement a double-check system for slide orientation
  • Train staff on proper handling techniques for slides
Equipment: Microscope slidesRelated SOP: Slide Preparation and Mounting SOP

This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.

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