Knowledge Base › Troubleshooting Atlas › Digital pathology
Out-of-focus regions on whole-slide images
What it looks like
Regions of the whole-slide image appear blurred or lacking detail, making it difficult to assess cellular structures.
Where it usually appears: Typically observed in areas of the slide that are not properly focused during scanning.
Likely causes
- Inadequate focus during scanning
- Slide thickness variation
- Incorrect Z-axis calibration
- Dirt or damage on the objective lens
Immediate checks
- Verify the focus setting of the scanner
- Inspect the slide for physical damage or uneven thickness
Corrective action
- Re-scan the slide with adjusted focus
- Clean the objective lens thoroughly
Preventive action
- Regular maintenance of the scanning equipment
- Ensure slides are prepared with consistent thickness
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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