PAS Staining SOP

Purpose

To provide a standardized method for performing Periodic Acid-Schiff (PAS) staining to identify polysaccharides in tissue samples.

Scope

This SOP applies to all histopathology laboratory personnel involved in staining procedures.

Responsibilities

• Technician: Prepare and perform PAS staining according to this SOP.
• Quality Manager: Ensure compliance with the SOP and maintain records.

Materials & equipment

• Periodic Acid solution
• Schiff's reagent
• Distilled water
• Microscope slides
• Cover slips
• Staining jars

Procedure

1. Prepare tissue sections on microscope slides.
2. Deparaffinize and hydrate sections through graded alcohols to water.
3. Immerse slides in Periodic Acid solution for 5-10 minutes.
4. Rinse slides in distilled water for 1-2 minutes.
5. Apply Schiff's reagent to the sections for 15-30 minutes in the dark.
6. Rinse slides in running tap water for 5 minutes.
7. Counterstain with hematoxylin for 1-2 minutes if desired.
8. Rinse slides in distilled water.
9. Dehydrate through graded alcohols and clear in xylene.
10. Mount with a cover slip and label the slides.

Records

• Staining log indicating date, operator, and samples stained.
• Quality control records for reagents used.

Safety

• Wear appropriate personal protective equipment (PPE) including gloves and lab coat.
• Handle all chemicals according to their safety data sheets (SDS).