Fixation SOP

Document: SOP-TEMPLATEVersion: 1.0 (template)Owner: [Your lab]

Purpose

To standardise fixation so morphology and antigenicity are preserved.

Scope

All specimens requiring formalin fixation.

Responsibilities

Grossing technician / pathologist: ensure adequate fixation before processing

Materials & equipment

10% neutral buffered formalin
Appropriately sized containers

Procedure

Place tissue in 10% NBF at a fixative-to-tissue ratio of about 10:1.
Slice large/thick specimens (bread-loaf) to allow penetration.
Record time in and out of fixative.
Ensure adequate fixation time appropriate to specimen size before processing.
Minimise cold-ischaemia time; fix promptly after removal.

Records

Fixation time log

Safety

Handle formalin in a fume hood with PPE; follow the formalin handling SOP.

Disclaimer

This is a template for educational and planning purposes. Laboratories must adapt it to their institutional SOPs, manufacturer instructions, applicable accreditation requirements, and pathologist approval before use.

Unimeditrek Pvt. Ltd. · www.unilabequipments.com · Educational template — adapt to your laboratory.

Unimeditrek supports laboratories with equipment, service and workflow planning. Talk to a specialist →