Knowledge Base › Troubleshooting Atlas › Quality & QC
Turnaround-time delays from re-cuts
What it looks like
Re-cuts may show uneven staining or poor morphology on slides. Blocks may appear to have irregular edges or inconsistencies.
Where it usually appears: Typically observed during histological examination when initial cuts are unsatisfactory.
Likely causes
- Inadequate fixation time
- Improper embedding technique
- Incorrect microtome settings
- Poor quality of tissue sample
- Inconsistent sectioning technique
Immediate checks
- Verify fixation time for samples
- Check embedding quality of blocks
- Inspect microtome blade sharpness
Corrective action
- Re-fix samples if fixation was inadequate
- Re-embed tissue samples properly
- Adjust microtome settings for optimal sectioning
Preventive action
- Implement standardized fixation protocols
- Train staff on embedding and sectioning techniques
- Regularly maintain and calibrate microtome equipment
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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