Knowledge Base › Troubleshooting Atlas › Embedding
Tubular structures not embedded on cross-section
What it looks like
Tubular structures appear distorted or not visible in cross-section on the slide or block.
Where it usually appears: Typically observed in tissue samples containing glandular or tubular structures, such as kidney or salivary gland.
Likely causes
- Inadequate fixation leading to tissue shrinkage
- Improper orientation of the tissue during embedding
- Insufficient embedding medium penetration
- Incorrect temperature during embedding
- Inadequate trimming of the block
Immediate checks
- Verify the orientation of the tissue in the embedding mold
- Check for proper fixation time and conditions
- Inspect the embedding medium for consistency
Corrective action
- Re-embed the tissue with proper orientation
- Adjust the trimming technique to expose tubular structures
- Ensure adequate infiltration of embedding medium
Preventive action
- Follow standardized fixation protocols
- Train staff on proper embedding techniques
- Regularly calibrate embedding equipment
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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