Knowledge Base › Troubleshooting Atlas › Tissue processing
Tissue shrinkage after processing
Also known as: tissue contraction, shrinkage artifact
What it looks like
Tissue appears smaller than expected; edges may be curled or distorted on the slide.
Where it usually appears: Commonly seen in paraffin-embedded sections during routine histological examination.
Likely causes
- Over-dehydration during processing
- Inadequate embedding medium
- Excessive heat during paraffin infiltration
- Improper fixation time
Immediate checks
- Verify processing times and temperatures
- Check for adequate fixation prior to processing
Corrective action
- Reprocess the tissue with adjusted dehydration steps
- Ensure proper embedding medium is used
Preventive action
- Monitor dehydration steps closely
- Use appropriate fixation protocols
- Maintain consistent processing temperatures
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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