Knowledge Base › Troubleshooting Atlas › Microtomy
Tissue crumbles away from the wax
What it looks like
Tissue appears fragmented or missing from the paraffin block. Sections may show gaps or voids where the tissue should be present.
Where it usually appears: Commonly observed during sectioning of poorly infiltrated or over-fixed tissue samples.
Likely causes
- Inadequate infiltration of tissue with paraffin wax
- Over-fixation leading to tissue brittleness
- Improper cooling of the paraffin block
- Incorrect microtome settings or blade angle
Immediate checks
- Inspect the infiltration time and temperature used for the tissue
- Check the microtome blade for damage or dullness
- Evaluate the cooling process of the paraffin block
Corrective action
- Re-infiltrate the tissue with fresh paraffin wax under optimal conditions
- Adjust microtome settings or replace the blade if necessary
- Use a softer cutting technique or adjust the angle of the blade
Preventive action
- Ensure proper fixation and infiltration protocols are followed
- Regularly inspect and maintain microtome equipment
- Train staff on proper handling of delicate tissues
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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