Knowledge Base › Troubleshooting Atlas › Digital pathology
Stitching artifacts across scan tiles
Also known as: stitching errors, tile misalignment
What it looks like
Visible lines or seams between adjacent scan tiles that disrupt the continuity of the image.
Where it usually appears: Commonly observed in large tissue sections or when multiple tiles are stitched together during digital scanning.
Likely causes
- Inconsistent focus across tiles
- Improper calibration of the scanner
- Variations in scanning speed or settings
Immediate checks
- Verify scanner calibration settings
- Inspect the scanned tiles for alignment issues
Corrective action
- Re-scan the affected areas with adjusted settings
- Manually correct stitching in image processing software
Preventive action
- Regularly calibrate the scanner before use
- Use consistent scanning parameters for all tiles
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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