Knowledge Base › Troubleshooting Atlas › Microtomy
Sections stick to the anti-roll plate or blade
Also known as: section adhesion, section sticking
What it looks like
Sections may appear wrinkled or torn on the slide, with visible remnants on the anti-roll plate or blade.
Where it usually appears: Typically observed during microtomy of paraffin-embedded tissues, especially with delicate samples.
Likely causes
- Insufficiently cooled paraffin blocks
- Too thick sections
- Dirty or damaged blade
- Improper blade angle
- Excessive blade pressure
Immediate checks
- Check the temperature of the paraffin block
- Inspect the blade for damage or debris
Corrective action
- Re-trim the block to achieve a better surface
- Replace or clean the blade
- Adjust the angle of the blade
Preventive action
- Ensure proper cooling of the block before sectioning
- Regularly clean the blade and anti-roll plate
- Train staff on proper microtomy techniques
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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