Knowledge Base › Troubleshooting Atlas › Microtomy
Sections show a venetian-blind (washboard) pattern
Also known as: washboard pattern
What it looks like
Sections exhibit a series of parallel grooves resembling a venetian blind. This pattern can obscure cellular details and affect diagnostic quality.
Where it usually appears: Commonly observed in thick sections or when cutting through dense tissue.
Likely causes
- Improper blade angle
- Dull or damaged microtome blade
- Inadequate tissue fixation
- Incorrect embedding technique
Immediate checks
- Inspect the microtome blade for damage
- Check the angle of the blade
- Evaluate the tissue embedding quality
Corrective action
- Replace or sharpen the microtome blade
- Adjust the blade angle
- Re-embed the tissue if necessary
Preventive action
- Regularly inspect and maintain microtome blades
- Use proper embedding techniques
- Ensure adequate fixation of tissues
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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