Knowledge Base › Troubleshooting Atlas › Floatation & mounting
Sections overlap on the slide
What it looks like
Sections appear layered or stacked on the slide, making it difficult to distinguish individual specimens.
Where it usually appears: Often seen during the mounting process or when preparing multiple sections from a single block.
Likely causes
- Inadequate warming of the slide surface prior to mounting
- Excessive thickness of the sections
- Improper handling during transfer from the water bath to the slide
- Inconsistent sectioning technique
Immediate checks
- Inspect the slide temperature to ensure it is adequately warmed
- Evaluate the section thickness using a micrometer
Corrective action
- Re-mount the sections with proper spacing and technique
- Use a heated slide warmer to facilitate proper adhesion
Preventive action
- Ensure consistent sectioning thickness during the cutting process
- Maintain optimal slide temperatures during mounting
- Train personnel on proper handling techniques for sections
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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