Knowledge Base › Troubleshooting Atlas › Microtomy
Sections alternating thick and thin
What it looks like
Sections exhibit alternating thickness, with some areas appearing thicker than others.
Where it usually appears: Typically observed during routine microtomy of paraffin-embedded tissue blocks.
Likely causes
- Inconsistent knife angle
- Improper tissue embedding
- Variable paraffin block temperature
- Inadequate knife sharpness
- Inconsistent cutting speed
Immediate checks
- Check knife angle and alignment
- Inspect the quality of the paraffin block
- Evaluate the microtome settings
Corrective action
- Adjust knife angle for consistency
- Re-embed tissue if necessary
- Sharpen or replace the microtome knife
Preventive action
- Regular maintenance of microtome
- Consistent embedding techniques
- Routine quality checks on knife sharpness
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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