Knowledge Base › Troubleshooting Atlas › Quality & QC
Section thickness not reproducible between staff
What it looks like
Sections appear uneven in thickness, leading to inconsistent staining and diagnostic quality.
Where it usually appears: Typically observed during routine microtomy when multiple staff members are cutting sections.
Likely causes
- Differences in cutting technique among staff
- Variability in microtome settings
- Inconsistent paraffin block quality
- Improper blade angle or dull blades
- Environmental factors like temperature and humidity
Immediate checks
- Verify microtome settings are consistent
- Check blade sharpness and condition
- Inspect paraffin blocks for uniformity
Corrective action
- Standardize training for all staff on microtomy techniques
- Implement a checklist for microtome setup before use
- Rotate staff through a single microtome to ensure consistency
Preventive action
- Regularly calibrate and maintain microtome equipment
- Conduct periodic training refreshers for staff
- Establish a quality control program for section thickness
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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