Knowledge Base › Troubleshooting Atlas › Microtomy
Ribbon splits into strips
What it looks like
The tissue ribbon appears fragmented and is not continuous, resembling strips rather than a smooth section.
Where it usually appears: Typically observed during sectioning of paraffin-embedded tissues.
Likely causes
- Blade dullness or damage
- Incorrect microtome settings
- Inadequate tissue fixation
- Improper embedding technique
Immediate checks
- Inspect the microtome blade for sharpness
- Verify microtome settings for thickness
- Check for any debris in the cutting area
Corrective action
- Replace or sharpen the microtome blade
- Adjust the microtome settings as necessary
- Re-embed the tissue block if fixation was inadequate
Preventive action
- Regularly check and maintain microtome blades
- Follow proper embedding protocols
- Ensure optimal fixation times and conditions
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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