Knowledge Base › Troubleshooting Atlas › Microtomy
Ribbon curls upward off the blade
Also known as: curling, upward ribbon
What it looks like
The tissue ribbon appears curled upwards off the blade, making it difficult to obtain uniform sections.
Where it usually appears: Typically observed during microtomy when cutting paraffin-embedded tissue blocks.
Likely causes
- Blade angle is incorrect
- Tissue block temperature is too warm
- Blade is dull or damaged
- Inadequate trimming of the block
- Excessive pressure during cutting
Immediate checks
- Check the blade angle and adjust if necessary
- Ensure the block is at the correct temperature before cutting
Corrective action
- Replace or sharpen the blade
- Trim the block more adequately before sectioning
- Adjust the cutting speed and pressure
Preventive action
- Regularly check and maintain blades
- Ensure proper embedding and cooling of tissue blocks
- Train staff on proper microtomy techniques
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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