Knowledge Base › Troubleshooting Atlas › Cytology preparation
Poor fixation of cytology smears
What it looks like
Cytology smears appear watery or have a washed-out appearance. Cellular details may be obscured or lost.
Where it usually appears: Typically seen in smears prepared from fresh specimens or during transport.
Likely causes
- Delayed fixation after specimen collection
- Inadequate volume of fixative used
- Improper technique during smear preparation
- Use of fixative that is not suitable for the specimen type
Immediate checks
- Check the time elapsed since specimen collection
- Inspect the fixative volume used
- Review the smear preparation technique
Corrective action
- Re-fix the smears in appropriate fixative
- Prepare new smears using proper technique and adequate fixative
- Ensure immediate fixation after collection
Preventive action
- Train staff on proper fixation protocols
- Implement a timer for fixation time
- Regularly check fixative quality and suitability
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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