Knowledge Base › Troubleshooting Atlas › Microtomy
Paraffin block cracks or splits on the surface
Also known as: block surface cracking, paraffin splitting
The surface of the paraffin block shows visible cracks or splits. This may affect the quality of the sections cut from the block.
Where it usually appears: Typically observed during the microtomy process or when handling the paraffin block prior to sectioning.
- Insufficient cooling of the block after embedding
- Rapid temperature changes during sectioning
- Inadequate infiltration of the tissue with paraffin
- Excessive force applied during sectioning
- Check the temperature of the microtome
- Inspect the block for proper embedding technique
- Evaluate the cooling time after embedding
- Allow the block to equilibrate at room temperature before sectioning
- Re-embed the tissue if infiltration was inadequate
- Ensure proper cooling of the block after embedding
- Maintain consistent temperature during sectioning
- Use appropriate embedding techniques to avoid air pockets
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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