Knowledge Base › Troubleshooting Atlas › Tissue processing
Overheated wax hardens or scorches tissue
Also known as: scorched tissue, hard wax
What it looks like
Tissue may appear burnt or discolored. Wax may be overly hard and brittle.
Where it usually appears: Typically seen during paraffin embedding if the wax temperature exceeds recommended levels.
Likely causes
- Wax temperature too high
- Insufficient cooling time for tissue
- Inappropriate wax type
Immediate checks
- Check wax temperature setting
- Inspect tissue for discoloration
- Evaluate cooling time
Corrective action
- Lower wax temperature
- Re-embed tissue in fresh wax
- Adjust embedding protocols
Preventive action
- Regularly calibrate temperature settings
- Use a thermometer to monitor wax temperature
- Follow SOP for embedding times
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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