Knowledge Base › Troubleshooting Atlas › Microtomy
Only partial sections of the tissue appear
Also known as: incomplete sections, partial cuts
What it looks like
Sections show only fragments of tissue instead of complete slices. The block may appear uneven or damaged.
Where it usually appears: Typically observed during routine microtomy when cutting paraffin-embedded tissue blocks.
Likely causes
- Block is too hard due to improper embedding or over-hardening
- Blade is dull or damaged
- Incorrect cutting angle or speed
- Inadequate support of the tissue block during cutting
- Tissue is not adequately infiltrated with paraffin
Immediate checks
- Inspect the microtome blade for sharpness and damage
- Check the embedding medium for proper consistency
- Examine the tissue block for signs of over-hardening
Corrective action
- Replace or sharpen the microtome blade
- Adjust cutting speed and angle
- Re-embed the tissue block if necessary
Preventive action
- Regularly check and replace microtome blades
- Ensure proper embedding techniques are followed
- Maintain consistent paraffin infiltration protocols
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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