Knowledge Base › Troubleshooting Atlas › IHC
IHC edge (rim) artifact
Also known as: rim artifact, edge effect
What it looks like
Thin, dark line or halo around the tissue section on the slide.
Where it usually appears: Typically observed at the edges of the tissue sections, especially in poorly fixed specimens.
Likely causes
- Inadequate fixation
- Overheating during processing
- Improper embedding technique
- Inconsistent sectioning thickness
- Contamination during staining
Immediate checks
- Inspect fixation time and quality
- Check for overheating during processing
Corrective action
- Re-fix the tissue specimen
- Adjust processing temperatures
- Re-embed and section the tissue properly
Preventive action
- Ensure optimal fixation protocols are followed
- Maintain consistent processing temperatures
- Train staff on proper embedding techniques
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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