Knowledge Base › Troubleshooting Atlas › Microtomy
Hard tissue deflects the blade (skip/jump lines)
Also known as: skip lines, jump lines
What it looks like
The section shows discontinuities or gaps in the cut surface, often appearing as lines or skips in the tissue.
Where it usually appears: Typically observed during the microtomy of hard tissues like bone or calcified specimens.
Likely causes
- Blade dullness or damage
- Incorrect blade angle
- Inadequate tissue fixation
- Improper embedding technique
- Inconsistent microtome settings
Immediate checks
- Inspect the blade for chips or dullness
- Verify the blade angle and alignment
- Check the embedding medium for consistency
Corrective action
- Replace or sharpen the blade
- Adjust the blade angle
- Re-embed the tissue if fixation is inadequate
- Calibrate the microtome settings
Preventive action
- Regularly check and maintain blades
- Use appropriate embedding techniques
- Ensure proper fixation of hard tissues
- Train staff on microtome operation
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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