Knowledge Base › Troubleshooting Atlas › Frozen section
Frozen sections tear or shred
What it looks like
Sections appear ragged or have uneven edges, with visible tearing or shredding. The tissue architecture is compromised, making it difficult to assess morphology.
Where it usually appears: Typically observed during the cutting of frozen sections, especially with delicate or fatty tissues.
Likely causes
- Improper freezing of the tissue sample
- Dull or damaged microtome blade
- Incorrect section thickness settings
- Inadequate support of the tissue block during cutting
- Excessive thawing of the tissue before sectioning
Immediate checks
- Inspect the microtome blade for damage or dullness
- Verify the freezing technique used for the sample
- Check the section thickness settings
Corrective action
- Replace or sharpen the microtome blade
- Re-freeze the tissue sample properly before cutting
- Adjust the section thickness to appropriate levels
Preventive action
- Ensure proper freezing techniques are followed
- Regularly maintain and calibrate the microtome
- Use appropriate embedding media to support delicate tissues
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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