Knowledge Base › Troubleshooting Atlas › Equipment faults
Cryostat frosting / icing up
Also known as: frost buildup, ice formation
What it looks like
Frost or ice crystals may be visible on the cryostat chamber or on the specimen block, affecting the quality of the sections.
Where it usually appears: Typically observed during prolonged use of the cryostat or in environments with high humidity.
Likely causes
- High ambient humidity
- Inadequate temperature settings
- Frequent door openings
- Improper maintenance of the cryostat
- Insufficient airflow around the cryostat
Immediate checks
- Check the temperature settings on the cryostat
- Inspect the door seal for damage
- Evaluate the room humidity levels
Corrective action
- Reduce humidity levels in the laboratory
- Adjust the cryostat temperature settings
- Limit door openings during operation
Preventive action
- Regular maintenance and servicing of the cryostat
- Monitor humidity and temperature in the laboratory
- Train staff on proper usage protocols
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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