Knowledge Base › Troubleshooting Atlas › Cytology preparation
Cell loss during liquid-based processing
Also known as: cell loss, sample degradation
What it looks like
Reduced cellularity on the slide, with fewer cells than expected. Possible obscuring of cellular details.
Where it usually appears: Typically observed during the preparation of cytology samples, especially in liquid-based cytology.
Likely causes
- Inadequate sample collection
- Improper fixation
- Insufficient centrifugation
- Excessive washing of the sample
- Old or degraded processing reagents
Immediate checks
- Review sample collection technique
- Check fixation time and method
- Examine centrifuge settings
Corrective action
- Repeat sample collection if possible
- Adjust centrifugation speed and time
- Optimize fixation protocol
Preventive action
- Train staff on proper collection techniques
- Regularly calibrate and maintain equipment
- Monitor reagent expiration dates
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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