Knowledge Base › Troubleshooting Atlas › Embedding
Block separates from the cassette base
What it looks like
The tissue block is detached from the cassette base, leading to potential loss of sample integrity. This may appear as a loose block or tissue floating away from the base during sectioning.
Where it usually appears: Often seen during embedding or when retrieving blocks from storage.
Likely causes
- Insufficient embedding medium used
- Inadequate cooling time before processing
- Improper cassette alignment during embedding
- Old or degraded embedding medium
Immediate checks
- Inspect the embedding medium for consistency
- Check the cassette for any visible defects
- Assess the cooling temperature and time used
Corrective action
- Re-embed the tissue block ensuring proper alignment
- Use fresh embedding medium
- Allow adequate cooling time before processing
Preventive action
- Regularly check and replace embedding medium
- Ensure proper procedural training for staff
- Maintain equipment to ensure optimal performance
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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