Knowledge Base › Troubleshooting Atlas › IHC
Batch-to-batch IHC variability
Also known as: IHC variability, staining inconsistency
What it looks like
Inconsistent staining intensity or pattern across different batches of slides.
Where it usually appears: Typically seen during the evaluation of IHC results across multiple runs or batches.
Likely causes
- Differences in antibody lot
- Variability in tissue fixation
- Inconsistent antigen retrieval
- Differences in incubation times
- Variations in wash steps
Immediate checks
- Verify antibody lot numbers
- Check tissue fixation protocols
- Assess antigen retrieval conditions
Corrective action
- Re-run with a single antibody lot
- Standardize fixation and retrieval protocols
- Adjust incubation times based on control results
Preventive action
- Implement strict quality control for reagents
- Maintain consistent fixation and processing protocols
- Conduct regular training for staff on IHC techniques
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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