Knowledge Base › Troubleshooting Atlas › Floatation & mounting
Air bubbles trapped under the section
What it looks like
Air bubbles appear as clear circles or voids beneath the tissue section on the slide.
Where it usually appears: Typically observed during the mounting process of the slide.
Likely causes
- Insufficient immersion in mounting medium
- Rapid application of mounting medium
- Inadequate warming of the slide
- Poor technique during slide preparation
Immediate checks
- Inspect the slide for visible bubbles
- Check the viscosity of the mounting medium
Corrective action
- Gently tap the slide to dislodge bubbles
- Reapply mounting medium if necessary
Preventive action
- Ensure proper technique during slide preparation
- Allow mounting medium to flow slowly over the section
This atlas is for educational purposes for laboratory professionals. It does not replace your institutional SOPs, manufacturer instructions, validated protocols or pathologist judgment. Adapt any action to your laboratory and equipment.
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