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NABL (ISO 15189) Readiness Checklist — Histopathology Laboratory

69 check-points · 11 sectionsLab: [Your laboratory]Assessed by: __________Date: __________

Use this self-assessment to prepare a histopathology / cytology laboratory for NABL accreditation under ISO 15189. Tick each item, add evidence references, and act on gaps.

1. Quality Management System

  • Quality manual in place and controlled
  • Quality policy and measurable quality objectives defined
  • Document control procedure (issue, review, versioning, obsolete removal)
  • Master list of SOPs; each SOP authorised, dated and version-controlled
  • Control of records procedure with defined retention
  • Scope of accreditation clearly defined for histopathology / cytology

2. Organisation & Personnel

  • Organisation chart and defined responsibilities
  • Job descriptions for pathologists, technologists and support staff
  • Qualification and registration records on file
  • Induction and ongoing training records
  • Documented competency assessment (initial + periodic)
  • Authorisation/signatory list for reporting
  • Confidentiality and impartiality declarations

3. Facilities & Safety

  • Adequate, segregated space (grossing, processing, microtomy, staining, reporting)
  • Ventilation / fume extraction at grossing and reagent areas
  • Temperature and humidity monitored where required
  • Biosafety and chemical safety risk assessments
  • PPE available and used; safety data sheets accessible
  • Biomedical waste segregation per BMW Rules 2016
  • Spill kits, first-aid and emergency procedures in place
  • Controlled access and specimen security

4. Equipment & Calibration

  • Equipment inventory with unique IDs
  • Installation / performance verification records
  • Calibration schedule and traceable calibration records (thermometers, balances, timers, microtomes, pipettes)
  • Preventive maintenance schedule and logs
  • Breakdown / service records and impact assessment
  • Temperature logs for fridges, freezers, water baths, ovens, wax baths
  • Equipment out-of-service labelling procedure

5. Pre-examination (Pre-analytical)

  • Test request form with adequate patient + clinical details
  • Two-identifier specimen labelling and matching
  • Specimen collection, fixation and transport instructions issued
  • Fixative type/ratio and fixation time recorded
  • Documented sample acceptance / rejection criteria
  • Accessioning with unique number and traceability
  • Cold-ischaemia / turnaround monitoring where relevant

6. Examination (Analytical)

  • Validated / verified SOPs for grossing, processing, embedding, microtomy, staining and IHC
  • Grossing performed/supervised by authorised staff with dictation records
  • H&E and special-stain control slides run and reviewed
  • IHC positive and negative controls with every batch
  • Antibody validation and optimisation records
  • Reagent and antibody lot-change verification
  • Uncertainty / limitations understood where applicable

7. Post-examination (Post-analytical)

  • Standardised report format with required elements
  • Report review and authorisation by a pathologist
  • Turnaround-time targets defined and monitored
  • Critical / significant value communication and record
  • Amended-report procedure with audit trail
  • Structured / synoptic reporting for cancers where applicable
  • Referral (second opinion) and specimen send-out procedure

8. Reagents & Consumables

  • Reagent inventory with lot numbers and expiry
  • Storage conditions monitored
  • New-lot / new-batch acceptance checks
  • Reagent preparation and QC records
  • Reagent change / rotation schedule with records

9. Quality Assurance (IQC & EQA)

  • Internal quality control (daily control slides, staining QC) with records
  • Participation in an EQA / proficiency-testing programme
  • EQA results reviewed; corrective action on poor performance
  • Inter-observer / peer review process (as applicable)
  • Quality-indicator monitoring (TAT, rejection rate, re-cut rate, discrepancies)

10. Continual Improvement

  • Internal audit programme and schedule
  • Non-conformance recording and control
  • Root-cause analysis and corrective/preventive action (CAPA)
  • Complaints handling procedure and records
  • Management review at defined intervals with actions
  • Risk management and opportunities for improvement

11. Records & Retention

  • Defined retention periods for reports, blocks, slides and records
  • Secure, retrievable archival of blocks and slides
  • Backup and confidentiality of electronic records (LIS)
  • Traceability from request → block → slide → report
Disclaimer

This checklist is an educational readiness aid and is a simplified interpretation. It does not replace the official NABL / ISO 15189 requirements, assessor guidance, or your accreditation body's current documents. Always refer to the latest official standards.

Unimeditrek Pvt. Ltd. · www.unilabequipments.com · Educational template — adapt to your laboratory.

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